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pc12 adh cells  (ATCC)


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    Structured Review

    ATCC pc12 adh cells
    Pc12 Adh Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc12+adh+cells/PC-12/pm38619592-167-0-23
    Average 98 stars, based on 4409 article reviews
    pc12 adh cells - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Neurite outgrowth resistance to rho kinase inhibitors in PC12 Adh cell.
    Article Snippet: PC12 cells were incubated in RPMI-1640 medium with 10% heat-inactivated horse serum and 5% fetal bovine serum in an atmosphere containing 5% carbon dioxide (CO2) and 95% air at 37 C (http://www.atcc.org/Products/All/CRL1721.aspx#culturemethod). .. PC12 Adh cells were incubated in Ham’s F12K medium with 15% horse serum and 2.5% fetal bovine serum in an atmosphere containing 5%CO2 and 95% air at 37 C (http://www.atcc.org/Products/All/CRL1721.1.aspx#culturemethod). ..

    Variant Assay:

    Article Title: Tl(I) and Tl(III)-induce genotoxicity, reticulum stress and autophagy in PC12 Adh cells.
    Article Snippet: Thallium (Tl) and its two cationic species, Tl(I) and Tl(III), are toxic for most living beings.. In this work, we investigated the effects of Tl (10–100 μM) on the viability and proliferation capacity of the adherent variant of PC12 cells (PC12 Adh cells).. While both Tl(I) and Tl(III) halted cell proliferation from 24 h of incubation, their viability was ~ 90% even after 72 h of treatment.

    Article Title: Selecting Improved Peptidyl Motifs for Cytosolic Delivery of Disparate Protein and Nanoparticle Materials
    Article Snippet: HEK 293T/17, COS-1, HeLa and A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, purchased from ATCC) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (ATCC) and 1% antibiotic/antimycotic (Sigma). .. PC12-Adh cells (an adherent variant of the parent PC12 cell line) were cultured in F-12K medium supplemented with 2.5% (v/v) heat-inactivated fetal bovine serum, 12.5% (v/v) horse serum (ATCC) and 1% (v/v) antibiotic/antimycotic. ..

    Derivative Assay:

    Article Title: Tl(I) and Tl(III)-induce genotoxicity, reticulum stress and autophagy in PC12 Adh cells.
    Article Snippet: Thallium (Tl) and its two cationic species, Tl(I) and Tl(III), are toxic for most living beings.. In this work, we investigated the effects of Tl (10–100 μM) on the viability and proliferation capacity of the adherent variant of PC12 cells (PC12 Adh cells).. While both Tl(I) and Tl(III) halted cell proliferation from 24 h of incubation, their viability was ~ 90% even after 72 h of treatment.

    Cell Culture:

    Article Title: Selecting Improved Peptidyl Motifs for Cytosolic Delivery of Disparate Protein and Nanoparticle Materials
    Article Snippet: HEK 293T/17, COS-1, HeLa and A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, purchased from ATCC) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (ATCC) and 1% antibiotic/antimycotic (Sigma). .. PC12-Adh cells (an adherent variant of the parent PC12 cell line) were cultured in F-12K medium supplemented with 2.5% (v/v) heat-inactivated fetal bovine serum, 12.5% (v/v) horse serum (ATCC) and 1% (v/v) antibiotic/antimycotic. ..

    Article Title: Novel multifunctional dopamine D 2 /D 3 receptors agonists with potential neuroprotection and anti-alpha synuclein protein aggregation properties.
    Article Snippet: Accepted Manuscript Novel multifunctional dopamine D2/D3 receptors agonists with potential neuroprotection and anti-alpha synuclein protein aggregation properties Dan Luo, Horrick Sharma, Deepthi Yedlapudi, Tamara Antonio, Maarten E.A.. Reith, Aloke K. Dutta PII: S0968-0896(16)30628-9 DOI: http://dx.doi.org/10.1016/j.bmc.2016.08.021 Reference: BMC 13204 To appear in: Bioorganic & Medicinal Chemistry Received Date: 1 June 2016 Revised Date: 15 August 2016 Accepted Date: 18 August 2016 Please cite this article as: Luo, D., Sharma, H., Yedlapudi, D., Antonio, T., Reith, M.E.A., Dutta, A.K., Novel multifunctional dopamine D2/D3 receptors agonists with potential neuroprotection and anti-alpha synuclein protein aggregation properties, Bioorganic & Medicinal Chemistry (2016), doi: http://dx.doi.org/10.1016/j.bmc.. 2016.08.021 This is a PDF file of an unedited manuscript that has been accepted for publication.

    other:

    Article Title: Optimisation of a PC12 cell-based in vitro stroke model for screening neuroprotective agents
    Article Snippet: PC12 Adh cells are often procured for its adherent phenotype, while ATCC provides no data on its differentiation.



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    Phototransfection Process and Visualization in Rat <t>PC12</t> Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
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    Phototransfection Process and Visualization in Rat <t>PC12</t> Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
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    Phototransfection Process and Visualization in Rat <t>PC12</t> Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
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    Phototransfection Process and Visualization in Rat <t>PC12</t> Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
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    ATCC pc12 adh adherent type rat pheochromocytoma cells
    Phototransfection Process and Visualization in Rat <t>PC12</t> Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
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    ATCC pc12 adh cell line
    Phototransfection Process and Visualization in Rat <t>PC12</t> Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
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    Image Search Results


    Phototransfection Process and Visualization in Rat PC12 Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.

    Journal: Frontiers in Drug Delivery

    Article Title: Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference

    doi: 10.3389/fddev.2024.1359700

    Figure Lengend Snippet: Phototransfection Process and Visualization in Rat PC12 Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.

    Article Snippet: The cell lines PC12 Adh (CRL-1721.1) and 3T3 (CRL-1658) were obtained from ATCC and cultured according to the provided protocol.

    Techniques: Fluorescence, Irradiation, Membrane

    Phototransfection of eGFP mRNA into Rat PC12 Cells, Human Astrocytes and Mouse 3T3 cells – (A) A phototransfected PC12 cell appears green from expression of eGFP protein after phototransfection with eGFP-encoding mRNA (0.38 μg/mL in the PBS surrounding the cell). Inset shows lack of fluorescence in nontransfected PC12 cells. (B) Two human astrocytes exhibit eGFP protein fluorescence after phototransfection with eGFP mRNA (0.38 μg/mL). Inset shows lack of fluorescence signal in nontransfected human astrocyte cells. (C) Mouse 3T3 cells also express eGFP after phototransfection (0.38 μg/mL). (D) Quantification of eGFP fluorescence data from multiple cell phototransfections. PC12 cells (N = 39) exhibited higher eGFP expression compared to human astrocytes (N = 59) and mouse 3T3 cells (N = 43). Data are presented as mean ± SEM. ***, p < 0.001. Scale bar is 45 microns.

    Journal: Frontiers in Drug Delivery

    Article Title: Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference

    doi: 10.3389/fddev.2024.1359700

    Figure Lengend Snippet: Phototransfection of eGFP mRNA into Rat PC12 Cells, Human Astrocytes and Mouse 3T3 cells – (A) A phototransfected PC12 cell appears green from expression of eGFP protein after phototransfection with eGFP-encoding mRNA (0.38 μg/mL in the PBS surrounding the cell). Inset shows lack of fluorescence in nontransfected PC12 cells. (B) Two human astrocytes exhibit eGFP protein fluorescence after phototransfection with eGFP mRNA (0.38 μg/mL). Inset shows lack of fluorescence signal in nontransfected human astrocyte cells. (C) Mouse 3T3 cells also express eGFP after phototransfection (0.38 μg/mL). (D) Quantification of eGFP fluorescence data from multiple cell phototransfections. PC12 cells (N = 39) exhibited higher eGFP expression compared to human astrocytes (N = 59) and mouse 3T3 cells (N = 43). Data are presented as mean ± SEM. ***, p < 0.001. Scale bar is 45 microns.

    Article Snippet: The cell lines PC12 Adh (CRL-1721.1) and 3T3 (CRL-1658) were obtained from ATCC and cultured according to the provided protocol.

    Techniques: Expressing, Fluorescence